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custom human cytokine antibody array kit  (Daemyung Co Ltd)

 
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    Daemyung Co Ltd custom human cytokine antibody array kit
    Custom Human Cytokine Antibody Array Kit, supplied by Daemyung Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+human+cytokine+antibody+array+kit/custom+human+cytokine+antibody+array+kit/pm23805819-95-1-7
    Average 90 stars, based on 1 article reviews
    custom human cytokine antibody array kit - by Bioz Stars, 2026-10
    90/100 stars

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    Ab Array:

    Article Title: Periodontal fibroblasts modulate proliferation and osteogenic differentiation of embryonic stem cells through production of fibroblast growth factors.
    Article Snippet: Background: Periodontal ligament fibroblasts (PLFs) maintain homeostasis of periodontal ligaments by producing paracrine factors that affect various functions of stem-like cells.. It is hypothesized that PLFs induce proliferation and differentiation of stem cells more effectively than gingival fibroblasts (GFs) and skin fibroblasts (SFs).. Methods: PLFs and GFs were isolated from extracted teeth and cultured in the presence and absence of osteogenesis-inducing factors.



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    RayBiotech inc customized human cytokine antibody array kit
    Inhibition of STAT3 increases production of NF-κB-dependent cytokines. <t>(A)</t> <t>LN229:EGFR,</t> U87:EGFR, and GBM8 cells were treated with DMSO or 3 μM Stattic for 24 h. Supernatant were prepared and used to assess 23 human cytokines with a human <t>cytokine</t> antibody array kit. GM-CSF, IL-6, IL-8, and control are boxed and labeled. Intensity of GM-CSF, IL6, and IL8 from LN229:EGFR cells was quantified by densitometry using a Silver Fast Scanner and ImageJ software. Data shown represent mean ± SD of quadruplicate measurements from two independent experiments. Fold changes were normalized to DMSO-treated control. ***P < 0.001, vehicle versus Stattic (GM-CSF); **P = 0.0011, vehicle versus Stattic (IL-6); no significant, vehicle versus Stattic (IL-8) by two-tailed Student’s t-test. (B) LN229:EGFR, U87:EGFR, and GBM8 cells were treated with 3 μM Stattic for 24 h, harvested, lysed, and analyzed by western blotting with indicated antibodies. For the expression levels of EGFR or GAPDH in each cell line, see Fig. 2A. (C) LN229:EGFR, U87:EGFR, and GBM8 cells were transfected with control siRNA or STAT3 siRNA for 48 h, harvested, lysed, and analyzed by western blot with indicated antibodies. For the expression levels of EGFR or GAPDH in each cell line, see Fig. 2B. (D) LN229:EGFR cells were treated with DMSO, 3 μM Stattic, 3 μM Bay11-7085, or Stattic plus Bay11-7085 for 24 h. These profiles obtained by incubating the array membranes with supernatant are shown. GM-CSF, IL-6, IL-8, and control are boxed and labeled. Intensity of GM-CSF, IL6, and IL8 was quantified by densitometry using a Silver Fast Scanner and ImageJ software. Data shown represent mean ± SD of quadruplicate measurements from 2 independent experiments. Fold changes were normalized to DMSO-treated control. For intensity of GM-CSF, ***P = 0.0003, vehicle versus Bay11-7085; ***P = 0.0002, vehicle versus Stattic; no significant, vehicle versus Bay-11-7085 plus Stattic. For intensity of IL6, *P = 0.0238, vehicle versus Bay11-7085; **P = 0.0012, vehicle versus Stattic; no significant, vehicle versus Bay-11-7085 plus Stattic. For intensity of IL8, **P = 0.0038, vehicle versus Bay11-7085; no significant, vehicle versus Stattic; **P = 0.0018, vehicle vs Bay-11-7085 plus Stattic by two-tailed Student’s t-test. (E) LN229:EGFR cells treated with 3 μM Stattic, 3 μM Bay11-7085, or 3 μM Stattic plus 3 μM Bay11-7085 for 24 h, harvested, lysed, and analyzed by western blot with indicated antibodies. (F) LN229:EGFR cells were treated with IL-6 (10 ng/mL), GM-CSF (1 ng/mL), or IL-8 (100 ng/mL) for indicated time (0.5–3 h), harvested, lysed, and analyzed by western blot with antibodies indicated. See also Supplementary Fig. 1.
    Customized Human Cytokine Antibody Array Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    customized human cytokine antibody array kit - by Bioz Stars, 2026-10
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    custom human cytokine antibody array kits  (RayBiotech inc)
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    Inhibition of STAT3 increases production of NF-κB-dependent cytokines. <t>(A)</t> <t>LN229:EGFR,</t> U87:EGFR, and GBM8 cells were treated with DMSO or 3 μM Stattic for 24 h. Supernatant were prepared and used to assess 23 human cytokines with a human <t>cytokine</t> antibody array kit. GM-CSF, IL-6, IL-8, and control are boxed and labeled. Intensity of GM-CSF, IL6, and IL8 from LN229:EGFR cells was quantified by densitometry using a Silver Fast Scanner and ImageJ software. Data shown represent mean ± SD of quadruplicate measurements from two independent experiments. Fold changes were normalized to DMSO-treated control. ***P < 0.001, vehicle versus Stattic (GM-CSF); **P = 0.0011, vehicle versus Stattic (IL-6); no significant, vehicle versus Stattic (IL-8) by two-tailed Student’s t-test. (B) LN229:EGFR, U87:EGFR, and GBM8 cells were treated with 3 μM Stattic for 24 h, harvested, lysed, and analyzed by western blotting with indicated antibodies. For the expression levels of EGFR or GAPDH in each cell line, see Fig. 2A. (C) LN229:EGFR, U87:EGFR, and GBM8 cells were transfected with control siRNA or STAT3 siRNA for 48 h, harvested, lysed, and analyzed by western blot with indicated antibodies. For the expression levels of EGFR or GAPDH in each cell line, see Fig. 2B. (D) LN229:EGFR cells were treated with DMSO, 3 μM Stattic, 3 μM Bay11-7085, or Stattic plus Bay11-7085 for 24 h. These profiles obtained by incubating the array membranes with supernatant are shown. GM-CSF, IL-6, IL-8, and control are boxed and labeled. Intensity of GM-CSF, IL6, and IL8 was quantified by densitometry using a Silver Fast Scanner and ImageJ software. Data shown represent mean ± SD of quadruplicate measurements from 2 independent experiments. Fold changes were normalized to DMSO-treated control. For intensity of GM-CSF, ***P = 0.0003, vehicle versus Bay11-7085; ***P = 0.0002, vehicle versus Stattic; no significant, vehicle versus Bay-11-7085 plus Stattic. For intensity of IL6, *P = 0.0238, vehicle versus Bay11-7085; **P = 0.0012, vehicle versus Stattic; no significant, vehicle versus Bay-11-7085 plus Stattic. For intensity of IL8, **P = 0.0038, vehicle versus Bay11-7085; no significant, vehicle versus Stattic; **P = 0.0018, vehicle vs Bay-11-7085 plus Stattic by two-tailed Student’s t-test. (E) LN229:EGFR cells treated with 3 μM Stattic, 3 μM Bay11-7085, or 3 μM Stattic plus 3 μM Bay11-7085 for 24 h, harvested, lysed, and analyzed by western blot with indicated antibodies. (F) LN229:EGFR cells were treated with IL-6 (10 ng/mL), GM-CSF (1 ng/mL), or IL-8 (100 ng/mL) for indicated time (0.5–3 h), harvested, lysed, and analyzed by western blot with antibodies indicated. See also Supplementary Fig. 1.
    Custom Human Cytokine Antibody Array Kits, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    custom human cytokine antibody array kit  (Daemyung Co Ltd)
    90
    Daemyung Co Ltd custom human cytokine antibody array kit
    Inhibition of STAT3 increases production of NF-κB-dependent cytokines. <t>(A)</t> <t>LN229:EGFR,</t> U87:EGFR, and GBM8 cells were treated with DMSO or 3 μM Stattic for 24 h. Supernatant were prepared and used to assess 23 human cytokines with a human <t>cytokine</t> antibody array kit. GM-CSF, IL-6, IL-8, and control are boxed and labeled. Intensity of GM-CSF, IL6, and IL8 from LN229:EGFR cells was quantified by densitometry using a Silver Fast Scanner and ImageJ software. Data shown represent mean ± SD of quadruplicate measurements from two independent experiments. Fold changes were normalized to DMSO-treated control. ***P < 0.001, vehicle versus Stattic (GM-CSF); **P = 0.0011, vehicle versus Stattic (IL-6); no significant, vehicle versus Stattic (IL-8) by two-tailed Student’s t-test. (B) LN229:EGFR, U87:EGFR, and GBM8 cells were treated with 3 μM Stattic for 24 h, harvested, lysed, and analyzed by western blotting with indicated antibodies. For the expression levels of EGFR or GAPDH in each cell line, see Fig. 2A. (C) LN229:EGFR, U87:EGFR, and GBM8 cells were transfected with control siRNA or STAT3 siRNA for 48 h, harvested, lysed, and analyzed by western blot with indicated antibodies. For the expression levels of EGFR or GAPDH in each cell line, see Fig. 2B. (D) LN229:EGFR cells were treated with DMSO, 3 μM Stattic, 3 μM Bay11-7085, or Stattic plus Bay11-7085 for 24 h. These profiles obtained by incubating the array membranes with supernatant are shown. GM-CSF, IL-6, IL-8, and control are boxed and labeled. Intensity of GM-CSF, IL6, and IL8 was quantified by densitometry using a Silver Fast Scanner and ImageJ software. Data shown represent mean ± SD of quadruplicate measurements from 2 independent experiments. Fold changes were normalized to DMSO-treated control. For intensity of GM-CSF, ***P = 0.0003, vehicle versus Bay11-7085; ***P = 0.0002, vehicle versus Stattic; no significant, vehicle versus Bay-11-7085 plus Stattic. For intensity of IL6, *P = 0.0238, vehicle versus Bay11-7085; **P = 0.0012, vehicle versus Stattic; no significant, vehicle versus Bay-11-7085 plus Stattic. For intensity of IL8, **P = 0.0038, vehicle versus Bay11-7085; no significant, vehicle versus Stattic; **P = 0.0018, vehicle vs Bay-11-7085 plus Stattic by two-tailed Student’s t-test. (E) LN229:EGFR cells treated with 3 μM Stattic, 3 μM Bay11-7085, or 3 μM Stattic plus 3 μM Bay11-7085 for 24 h, harvested, lysed, and analyzed by western blot with indicated antibodies. (F) LN229:EGFR cells were treated with IL-6 (10 ng/mL), GM-CSF (1 ng/mL), or IL-8 (100 ng/mL) for indicated time (0.5–3 h), harvested, lysed, and analyzed by western blot with antibodies indicated. See also Supplementary Fig. 1.
    Custom Human Cytokine Antibody Array Kit, supplied by Daemyung Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+human+cytokine+antibody+array+kit/custom+human+cytokine+antibody+array+kit/pm23805819-95-1-7
    Average 90 stars, based on 1 article reviews
    custom human cytokine antibody array kit - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    custom-designed membrane-based human cytokine antibody array kit  (RayBiotech inc)
    90
    RayBiotech inc custom-designed membrane-based human cytokine antibody array kit
    Inhibition of STAT3 increases production of NF-κB-dependent cytokines. <t>(A)</t> <t>LN229:EGFR,</t> U87:EGFR, and GBM8 cells were treated with DMSO or 3 μM Stattic for 24 h. Supernatant were prepared and used to assess 23 human cytokines with a human <t>cytokine</t> antibody array kit. GM-CSF, IL-6, IL-8, and control are boxed and labeled. Intensity of GM-CSF, IL6, and IL8 from LN229:EGFR cells was quantified by densitometry using a Silver Fast Scanner and ImageJ software. Data shown represent mean ± SD of quadruplicate measurements from two independent experiments. Fold changes were normalized to DMSO-treated control. ***P < 0.001, vehicle versus Stattic (GM-CSF); **P = 0.0011, vehicle versus Stattic (IL-6); no significant, vehicle versus Stattic (IL-8) by two-tailed Student’s t-test. (B) LN229:EGFR, U87:EGFR, and GBM8 cells were treated with 3 μM Stattic for 24 h, harvested, lysed, and analyzed by western blotting with indicated antibodies. For the expression levels of EGFR or GAPDH in each cell line, see Fig. 2A. (C) LN229:EGFR, U87:EGFR, and GBM8 cells were transfected with control siRNA or STAT3 siRNA for 48 h, harvested, lysed, and analyzed by western blot with indicated antibodies. For the expression levels of EGFR or GAPDH in each cell line, see Fig. 2B. (D) LN229:EGFR cells were treated with DMSO, 3 μM Stattic, 3 μM Bay11-7085, or Stattic plus Bay11-7085 for 24 h. These profiles obtained by incubating the array membranes with supernatant are shown. GM-CSF, IL-6, IL-8, and control are boxed and labeled. Intensity of GM-CSF, IL6, and IL8 was quantified by densitometry using a Silver Fast Scanner and ImageJ software. Data shown represent mean ± SD of quadruplicate measurements from 2 independent experiments. Fold changes were normalized to DMSO-treated control. For intensity of GM-CSF, ***P = 0.0003, vehicle versus Bay11-7085; ***P = 0.0002, vehicle versus Stattic; no significant, vehicle versus Bay-11-7085 plus Stattic. For intensity of IL6, *P = 0.0238, vehicle versus Bay11-7085; **P = 0.0012, vehicle versus Stattic; no significant, vehicle versus Bay-11-7085 plus Stattic. For intensity of IL8, **P = 0.0038, vehicle versus Bay11-7085; no significant, vehicle versus Stattic; **P = 0.0018, vehicle vs Bay-11-7085 plus Stattic by two-tailed Student’s t-test. (E) LN229:EGFR cells treated with 3 μM Stattic, 3 μM Bay11-7085, or 3 μM Stattic plus 3 μM Bay11-7085 for 24 h, harvested, lysed, and analyzed by western blot with indicated antibodies. (F) LN229:EGFR cells were treated with IL-6 (10 ng/mL), GM-CSF (1 ng/mL), or IL-8 (100 ng/mL) for indicated time (0.5–3 h), harvested, lysed, and analyzed by western blot with antibodies indicated. See also Supplementary Fig. 1.
    Custom Designed Membrane Based Human Cytokine Antibody Array Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+human+cytokine+antibody+array+kit/cytokine+array/pmc03890700-153-16-22
    Average 90 stars, based on 1 article reviews
    custom-designed membrane-based human cytokine antibody array kit - by Bioz Stars, 2026-10
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    Image Search Results


    Inhibition of STAT3 increases production of NF-κB-dependent cytokines. (A) LN229:EGFR, U87:EGFR, and GBM8 cells were treated with DMSO or 3 μM Stattic for 24 h. Supernatant were prepared and used to assess 23 human cytokines with a human cytokine antibody array kit. GM-CSF, IL-6, IL-8, and control are boxed and labeled. Intensity of GM-CSF, IL6, and IL8 from LN229:EGFR cells was quantified by densitometry using a Silver Fast Scanner and ImageJ software. Data shown represent mean ± SD of quadruplicate measurements from two independent experiments. Fold changes were normalized to DMSO-treated control. ***P < 0.001, vehicle versus Stattic (GM-CSF); **P = 0.0011, vehicle versus Stattic (IL-6); no significant, vehicle versus Stattic (IL-8) by two-tailed Student’s t-test. (B) LN229:EGFR, U87:EGFR, and GBM8 cells were treated with 3 μM Stattic for 24 h, harvested, lysed, and analyzed by western blotting with indicated antibodies. For the expression levels of EGFR or GAPDH in each cell line, see Fig. 2A. (C) LN229:EGFR, U87:EGFR, and GBM8 cells were transfected with control siRNA or STAT3 siRNA for 48 h, harvested, lysed, and analyzed by western blot with indicated antibodies. For the expression levels of EGFR or GAPDH in each cell line, see Fig. 2B. (D) LN229:EGFR cells were treated with DMSO, 3 μM Stattic, 3 μM Bay11-7085, or Stattic plus Bay11-7085 for 24 h. These profiles obtained by incubating the array membranes with supernatant are shown. GM-CSF, IL-6, IL-8, and control are boxed and labeled. Intensity of GM-CSF, IL6, and IL8 was quantified by densitometry using a Silver Fast Scanner and ImageJ software. Data shown represent mean ± SD of quadruplicate measurements from 2 independent experiments. Fold changes were normalized to DMSO-treated control. For intensity of GM-CSF, ***P = 0.0003, vehicle versus Bay11-7085; ***P = 0.0002, vehicle versus Stattic; no significant, vehicle versus Bay-11-7085 plus Stattic. For intensity of IL6, *P = 0.0238, vehicle versus Bay11-7085; **P = 0.0012, vehicle versus Stattic; no significant, vehicle versus Bay-11-7085 plus Stattic. For intensity of IL8, **P = 0.0038, vehicle versus Bay11-7085; no significant, vehicle versus Stattic; **P = 0.0018, vehicle vs Bay-11-7085 plus Stattic by two-tailed Student’s t-test. (E) LN229:EGFR cells treated with 3 μM Stattic, 3 μM Bay11-7085, or 3 μM Stattic plus 3 μM Bay11-7085 for 24 h, harvested, lysed, and analyzed by western blot with indicated antibodies. (F) LN229:EGFR cells were treated with IL-6 (10 ng/mL), GM-CSF (1 ng/mL), or IL-8 (100 ng/mL) for indicated time (0.5–3 h), harvested, lysed, and analyzed by western blot with antibodies indicated. See also Supplementary Fig. 1.

    Journal: Neuro-Oncology

    Article Title: Betacellulin drives therapy resistance in glioblastoma

    doi: 10.1093/neuonc/noz206

    Figure Lengend Snippet: Inhibition of STAT3 increases production of NF-κB-dependent cytokines. (A) LN229:EGFR, U87:EGFR, and GBM8 cells were treated with DMSO or 3 μM Stattic for 24 h. Supernatant were prepared and used to assess 23 human cytokines with a human cytokine antibody array kit. GM-CSF, IL-6, IL-8, and control are boxed and labeled. Intensity of GM-CSF, IL6, and IL8 from LN229:EGFR cells was quantified by densitometry using a Silver Fast Scanner and ImageJ software. Data shown represent mean ± SD of quadruplicate measurements from two independent experiments. Fold changes were normalized to DMSO-treated control. ***P < 0.001, vehicle versus Stattic (GM-CSF); **P = 0.0011, vehicle versus Stattic (IL-6); no significant, vehicle versus Stattic (IL-8) by two-tailed Student’s t-test. (B) LN229:EGFR, U87:EGFR, and GBM8 cells were treated with 3 μM Stattic for 24 h, harvested, lysed, and analyzed by western blotting with indicated antibodies. For the expression levels of EGFR or GAPDH in each cell line, see Fig. 2A. (C) LN229:EGFR, U87:EGFR, and GBM8 cells were transfected with control siRNA or STAT3 siRNA for 48 h, harvested, lysed, and analyzed by western blot with indicated antibodies. For the expression levels of EGFR or GAPDH in each cell line, see Fig. 2B. (D) LN229:EGFR cells were treated with DMSO, 3 μM Stattic, 3 μM Bay11-7085, or Stattic plus Bay11-7085 for 24 h. These profiles obtained by incubating the array membranes with supernatant are shown. GM-CSF, IL-6, IL-8, and control are boxed and labeled. Intensity of GM-CSF, IL6, and IL8 was quantified by densitometry using a Silver Fast Scanner and ImageJ software. Data shown represent mean ± SD of quadruplicate measurements from 2 independent experiments. Fold changes were normalized to DMSO-treated control. For intensity of GM-CSF, ***P = 0.0003, vehicle versus Bay11-7085; ***P = 0.0002, vehicle versus Stattic; no significant, vehicle versus Bay-11-7085 plus Stattic. For intensity of IL6, *P = 0.0238, vehicle versus Bay11-7085; **P = 0.0012, vehicle versus Stattic; no significant, vehicle versus Bay-11-7085 plus Stattic. For intensity of IL8, **P = 0.0038, vehicle versus Bay11-7085; no significant, vehicle versus Stattic; **P = 0.0018, vehicle vs Bay-11-7085 plus Stattic by two-tailed Student’s t-test. (E) LN229:EGFR cells treated with 3 μM Stattic, 3 μM Bay11-7085, or 3 μM Stattic plus 3 μM Bay11-7085 for 24 h, harvested, lysed, and analyzed by western blot with indicated antibodies. (F) LN229:EGFR cells were treated with IL-6 (10 ng/mL), GM-CSF (1 ng/mL), or IL-8 (100 ng/mL) for indicated time (0.5–3 h), harvested, lysed, and analyzed by western blot with antibodies indicated. See also Supplementary Fig. 1.

    Article Snippet: EGFR Ligand Antibody Array To study the secretion/expression of EGFR ligands, we used a customized human cytokine antibody array kit (RayBiotech).

    Techniques: Inhibition, Ab Array, Labeling, Software, Two Tailed Test, Western Blot, Expressing, Transfection